Journal: Bone & Joint Research
Article Title: The degenerated glenohumeral joint
doi: 10.1302/2046-3758.1310.BJR-2024-0026.R1
Figure Lengend Snippet: Immunohistochemical (IHC) scores of degeneration markers in humeral cartilage from omarthrosis (OmA), cuff tear arthropathy (CTA), and controls. Osteochondral specimens from five OmA and five CTA patients were selected that exhibited both mildly (Mankin score (MS) ≤ 7) and severely degraded (MS ≥ 8) regions. Intact cartilage regions were included from control specimens for comparison. All samples were processed for histological staining with a) Safranin O or b) to f) IHC staining with specific antibodies against b) collagen type I, c) collagen type II, d) the C1,2C neoepitope, e) collagen type X, and f) osteocalcin. *p < 0.05 vs control (Mann-Whitney U test for unpaired groups). #p < 0.05: MS < 7 vs MS > 8 (Wilcoxon test for paired groups). p-values between 0.05 and 0.1 are given in exact numbers.
Article Snippet: Immunohistochemical staining using antibodies against collagen type I (#1310-01, Southern Biotech, USA), collagen type II (#1320-01, Southern Biotech), collagen neoepitope C1,2C (#50-1035; IBEX, USA), collagen type X (#58632; Abcam, UK), and osteocalcin (#MAB1419; R&D Systems, USA) was performed using deparaffinized osteochondral tissue sections.
Techniques: Immunohistochemical staining, Control, Comparison, Staining, Immunohistochemistry, MANN-WHITNEY